surface plasmon resonance spr analysis spr  (Biacore)

Journal: Science Advances

Article Title: Beta cell–targeted PD-1 agonist inhibits cell-mediated autoimmunity in pancreas tissue slices

doi: 10.1126/sciadv.aec9029

Figure Lengend Snippet: ( A ) CF647-labeled ImmTAAI molecules were assessed for pHLA binding and compared to unlabeled molecules by SPR using BIAcore 8K. Binding to PPI or Tel cognate pHLA was carried out at 37°C. ( B ) Similarly, CF647-labeled ImmTAAI molecules were assessed for PD-1 binding and compared to unlabeled molecules. PD-1 binding was carried out at 25°C. ( C ) Schematic of the ECN90 beta cell line: Jurkat NFL Mel5 PD-1 reporter assay. ( D ) ECN90 or NCI-H1703 cells were pulsed with Melan-A–activating peptide, and titrations of unlabeled or labeled PPI ImmTAAI molecules were added.

Article Snippet: SPR binding analysis was performed using a BIAcore 8 K (GE Healthcare) as described previously ( ).

Techniques: Labeling, Binding Assay, Reporter Assay